%0 Journal Article %A Ebrahimzadeh Bideskan, Alireza %A Nikmard, Fatemeh %A Mohammadzadeh, Elham %A Razavi, Hengameh %A Fazel, Alireza %T Lectinhistochemistry Study of Glycoconjugate Terminal Sugars During Rat kidney Development %J Internal Medicine Today %V 18 %N 1 %U http://imtj.gmu.ac.ir/article-1-1502-en.html %R %D 2012 %K Embryonic development, glycoconjugates, kidney, lectin, %X Abstract Background and Aim: Developmental processes are mediated by cell surface and extracellular matrix (ECM) interactions that are regulated by the molecular mechanism. The aim of this study was to determine Glycoconjugates terminal sugars distribution and the changes during kidney development in rats using Lectinhistochemical technique. Materials and Methods: Thirty Wistar Rat embryos at different stages from days 14 (E14) to 18 days of gestation were fixed in formalin, embedded in paraffin and cut into 5µm thickness sections serially. The sections were incubated with different HRP-lectins from Lotus tetragonolobus (LTA), Maclura pomifera (MPA) and Ulex europeus agglutinine (UEA-I). Staining intensity was determined by means of blind method and the sections were graded to compare different embryonic stages. Results: Our findings showed that the reactions of MPA with podocyts started at E14 and increased afterwards (p %> http://imtj.gmu.ac.ir/article-1-1502-en.pdf %P 26-36 %& 26 %! %9 Applicable %L A-10-8-4 %+ %G eng %@ 2981-0086 %[ 2012