Aims:Millions of people consume psychoactives such as marijuana. Mesenchyme cells, which are derived from bone marrow stem cells, have multiple applications in tissue engineering and regenerative medicine. The aim of this study was to analyze the effects of marijuana on differentiation power of bone marrow stem cells of adult male rats in adipose tissues.

Materials and Methods: In this experimental study, the bone marrow stem cells of adult male rats were segregated with flushing method. Then, their mesenchymal identity was confirmed with a morphological study, PCR-RT method, and surface markers of CD45 and CD34 along with the related genes of CD90 and CD73. For analyzing the viability and differentiation power of the cells in adipose tissues in terms of marijuana usage, cell samples were exposed to different dosages of marijuana, such as 30 and 6000 ng/ml and cell survival was evaluated by MTT.The adipogenic differentiation potential of the treated cells were analyzed based on oil red staining. The results were analyzed by ANOVA and Schaffe tests.

Findings:The results indicated that the extracted cells are non-adherent in flasks and, in moving from passage one to the next passage, their duplication speed increased. In passage three, they changed into fusiform cells. Based on RT-PCR, it was revealed that CD90 and CD73 were existent, but CD34 and CD45 are nonexistent. Marijuana treatment caused no change in the form of stem cells. 10days after exposure to adipogenic circumstances, in addition to increase of survival, their cytoplasm was repleted with adipose.

Conclusion:The results of this study revealed that marijuana has positive effects on mesenchymal bone marrow stem cells in differentiating the adipocyte.