Volume 21, Issue 2 (Summer 2015)                   Intern Med Today 2015, 21(2): 91-96 | Back to browse issues page


XML Persian Abstract Print


1- Microbiology Department, Veterinary Faculty, Urmia University, Urmia, Iran , n.a.ahangran@gmail.com
2- Microbiology Department, Veterinary Faculty, Urmia University, Urmia, Iran
Abstract:   (4598 Views)

Aims: Bone marrow-derived mesenchymal stem cells have therapeutic potentials due to their immunomodulatory properties. Estrogen is also an immunomodulator. The current study was to analyze the effect of bone marrow-derived mesenchymal stem cells’ supernatant of male rats adjacent with estrogen on the function and survival of neutrophils. Materials & Methods: In this experimental study, mesenchymal stem cells isolated from the femur and tibia of the bone marrow of male 6-8 week old rats were cultured in DMEM. After maturation, the supernatant of the mesenchymal stem cells treated with estrogen (10nM and 20nM) and cultured for 72 hours at 37°C. Then, the mesenchymal stem cells were co-cultured with the peripheral blood neutrophils and the neutrophil functions were measured by phagocytosis and respiratory burst (Nitro Blue Tetrazolium resuscitation) tests. The viability of neutrophils was measured with acridine-orange fluorescent staining. Data were analyzed by SPSS 18 software, using independent T, one way ANOVA and Tukey tests. Findings: The respiratory burst in the groups treated with 10nM and 20nM of estrogen showed significant difference compared with the control group. The percentage of phagocytosis in the groups treated with 10nM and 20nM of estrogen were significantly increased compared with the control group. There was a significant difference between the percentage of phagocytosis of 10nM and 20nm groups (p<0.05). The apoptosis level in the groups treated with 10nM and 20nM of estrogen showed significant difference compared with the control group. Conclusion: Supernatant of mesenchymal stem cells treated with estrogen increases the phagocytosis potential and respiratory explosion of neutrophils.

Full-Text [PDF 427 kb]   (1534 Downloads)    
Type of Study: Original | Subject: Basic Medical Science
Received: 2014/11/25 | Accepted: 2015/05/11 | Published: 2015/05/24

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.