Volume 21, Issue 1 (Spring 2015)                   Intern Med Today 2015, 21(1): 13-19 | Back to browse issues page


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1- Biochemistry Department, Medicine Faculty, Baqiyatallah University of Medical Sciences, Tehran, Iran
2- Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran , Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
3- Students Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Abstract:   (5423 Views)

Aims: Organophosphates such as diazinon (DZN) induce the production of free radicals and oxidative stress. The aim of this study was to evaluate the role of vitamins E and C as antioxidants in reduction of DZN-induced oxidative stress in rat heart. Materials & Methods: In present experimental study, 36 male Wistar rats were randomly divided into six groups control (corn oil as DZN solvent), DZN (100mg/kg), vitamin E (150mg/kg), vitamin C (200mg/kg), vitamin E+DZN and vitamin C+DZN. 24 hours after intraperitoneal injection, animals were anesthetized by ether, and heart tissue was quickly removed. After tissues hemogenation, superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST) and lactate dehydrogenase (LDH) activities, as well as glutathione (GSH) and malondialdehyde (MDA) levels were determined by biochemical methods. The data were statistically analyzed using Instat 3.3 by analysis of variance (ANOVA) followed by post hoc Tukey test. Findings: DZN increased SOD, CAT and GST activities (p<0.01) and MDA level (p<0.05), while decreased LDH activity (p<0.05) and GSH content (p<0.01) in heart compared with the control group. Administration of vitamins E and C inhibited changing of these parameters. Conclusion: Administration of vitamins E and C as antioxidant decreases DZN-induced oxidative stress by scavenging free radicals.

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Type of Study: Original | Subject: Basic Medical Science
Received: 2014/11/12 | Accepted: 2015/01/31 | Published: 2015/03/17

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