Volume 16, Issue 2 (10-2010)                   Horizon Med Sci 2010, 16(2): 18-23 | Back to browse issues page

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Goudarzi H, Jahani Sherafat S, Farnia P, Mirsamadi E S. Identification of Beijing Strains of Mycobacterium Tuberculosis in Pulmonary Tuberculosis Patients with Culture Positive Specimens Using Multiplex PCR Method. Horizon Med Sci. 2010; 16 (2) :18-23
URL: http://hms.gmu.ac.ir/article-1-896-en.html
Abstract:   (9063 Views)

  Background and Aim: The genotype of Beijing strain of Mycobacterium tuberculosis has attracted special attention due to the association with multi drug resistance and rapid transmission although Spoligotyping is the gold standard for the identification and classification of Beijing strains of MTB. This technique however, is needed to apply special equipments that do not exist in clinical laboratory. Therefore, in this study we used a fast and cost-effective method for detection of this genotype to prevent its spread in the community.

  Materials and Methods: CTAB method was used to extract DNA from positive culture specimens in tuberculosis patients. Next, with spoligotyping, we determined different strains of MTB, and then we used multiplex PCR with 3 sets of PCR primers.

  Result: A total of 200 MTB isolates were genotyped by both spoligotyping and multiplex PCR of which19 isolates were determined to be Beijing strains and the remaining (181) isolates were non-Beijing strains. The multiplex PCR method indicated the same result.

  Conclusion: Considering the same detection power of two methods to distinguish Beijing strain and higher cost-effectiveness in comparison to spoligotyping, Multiplex PCR can be used in clinical laboratory settings.

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Type of Study: Original | Subject: Basic Medical Science
Received: 2010/10/10 | Published: 2010/10/15

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