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Horizon Med Sci 2017, 23(2): 149-155 Back to browse issues page
Effect of Aqueous Extract of Launaea acanthodes on DNA Oxidative Damage and Antioxidant Enzymes Activities in Diabetic Rats Testes Tissue
S. Tafakkor1, D. Sadoughi *2, R. Rahbarian1
1- Biology Department, Sciences Faculty, Tehran Branch, Payam-e-Noor University, Tehran, Iran
2- Young Researchers and Elite Club, Mashhad Branch, Islamic Azad University, Mashhad, Iran , damoon.sadughi@gmail.com
Abstract:   (200 Views)

Aims: Oxidative stress plays an important role in the initiation and progression of diabetes. Noticing the antioxidant and antidiabetes effects of Launaea acanthodes, the aim of the study was to determine the effects of the aqueous extract of the herb on DNA oxidative damage and the activities of antioxidant enzyms in the testicular tissue of diabetic rats. 

Materials & Methods: In the experimental study, 32 male Wistar rats were studied. The rats were randomly divided into four 8-rat groups including healthy control, diabetic control, and experimental diabetic groups treated by aqueous extract of Launaea acanthodes. Diabetes was induced in diabetic control and diabetic experimental groups via one alloxan intraperitoneal injection. 100 and 200mg/Kg aqueous extract of Launaea acanthodes were injected as intraperitoneal in diabetic experimental groups at alternate days for one month, while the control groups received only strile distilled water via injection. At the end of the study, the activities of antioxidant enzyms and the levels of 8-hydroxydeoxyguanosine and malondialdehyde were assessed in the testicular tissue. Data was analyzed by SPSS 20 software using one-way ANOVA and Tukey’s post-hoc tests. 

Findings: The tissue activities of superoxide dismutase, glutathione peroxidase, and catalase enzymes significantly increased in the experimental groups treated by 100 and 200mg/Kg aqueous extract of Launaea acanthodes as dose-dependent compared to diabetic control group, while the levels of 8-hydroxideoxiguanosine and malondialdehyde significantly decreased (p<0.05).

Conclusion: Dose-dependent administration of aqueous extract of Launaea acanthodes increases the antioxidant capacity, while reducing lipidic peroxidation and DNA oxidative damage in the testicular tissue of rats.

Keywords: Diabetes Mellitus [http://www.ncbi.nlm.nih.gov/mesh/68003920], Launaea acanthodes [Not in MeSH], Testis [http://www.ncbi.nlm.nih.gov/mesh/68013737], Oxidative Stress [http://www.ncbi.nlm.nih.gov/mesh/68018384], DNA [http://www.ncbi.nlm.nih.gov/mesh/68004247]
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Type of Study: Original | Subject: Basic Medical Science
Received: 2016/06/14 | Accepted: 2016/07/25 | Published: 2017/03/11
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DOI: 10.18869/acadpub.hms.23.2.149


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Tafakkor S, Sadoughi D, Rahbarian R. Effect of Aqueous Extract of Launaea acanthodes on DNA Oxidative Damage and Antioxidant Enzymes Activities in Diabetic Rats Testes Tissue. Horizon Med Sci . 2017; 23 (2) :149-155
URL: http://hms.gmu.ac.ir/article-1-2534-en.html
Volume 23, Number 2 (Spring 2017) Back to browse issues page
مجله علمی پژوهشی افق دانش The Horizon of Medical Sciences
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