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Horizon Med Sci 2015, 21(4): 7-11 Back to browse issues page
Polyethylene Glycol 200; a Rapid and Inexpensive Method for DNA Extraction from Gram Positive and Gram Negative Bacteria
J Mardaneh1, AR Mohammadzadeh *2, Z Masomian3
1- PhD Microbiology Department, Medicine School, Gonabad University of Medical Sciences, Gonabad, Iran
2- PhD Microbiology Department, Medicine School, Gonabad University of Medical Sciences, Gonabad, Iran , alm13604@gmail.com
3- BSc Student Research Committee, Gonabad University of Medical Sciences, Gonabad, Iran
Abstract:   (637 Views)

Aims: In general, DNA extraction from the gram-positive bacteria is too hard and expensive. The aim of this study was to utilize Polyethylene Glycol 200 in order to extract DNA from Lactobacillus acidophilus gram-positive bacteria and Pseudomonas aeruginosa gram-negative bacteria, as well as PCR conducting on them to search Lacto and ExoA genes, respectively.

Materials & Methods: Standard strains of Lactobacillus acidophilus gram-positive bacteria and Pseudomonas aeruginosa gram-negative bacteria were cultured on MRS and Mueller-Hinton agar, respectively. Then, the bacteria colony was dissolved in TE buffer and DNA was extracted using PEG 200. PCR reactions were done on the specific Lacto and ExoA genes of each organism.  

Findings: PCR was done on the selected genes for each organism; and bp231 Lacto genes were detected for the standard strain of Lactobacillus acidophilus and the strain of Lactobacillus acidophilus separated from the dairy. In addition, bp396 ExoA genes were detected for the standard strain of Pseudomonas aeruginosa and the clinical strains of Pseudomonas aeruginosa on agarose gel.

Conclusion: Since DNA extraction from gram-positive bacteria is too hard due their very strong walls, PEG 200 might be a very proper, affordable, quick, and available method to extract DNA from the gram-positive bacteria. In addition, DNA of gram-negative bacteria and fungi can simply be extracted through the method.

Keywords: Polyethylene Glycol 200, DNA Extraction, Gram-Positive Bacteria, Gram-Negative Bacteria
Full-Text [PDF 304 kb]   (357 Downloads)    
Type of Study: Original | Subject: Laboratory Medicine
Received: 2016/01/24 | Accepted: 2016/04/19 | Published: 2016/04/20
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Mardaneh J, Mohammadzadeh A, Masomian Z. Polyethylene Glycol 200; a Rapid and Inexpensive Method for DNA Extraction from Gram Positive and Gram Negative Bacteria. Horizon Med Sci . 2015; 21 (4) :7-11
URL: http://hms.gmu.ac.ir/article-1-2438-en.html
Volume 21, Number 4 (Special Issue 2015) Back to browse issues page
مجله علمی پژوهشی افق دانش The Horizon of Medical Sciences
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