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Horizon Med Sci 2016, 22(1): 1-11 Back to browse issues page
Effect of Aqueous Extract of Launaea acanthodes on Open Skin Wound in Diabetic Rats
Rahbarian R.1, Sepehri Moghadam H.2, Sadoughi S.D. *3
1- PhD Biology Department, Sciences Faculty, Payam-e-Noor University, Tehran, Iran
2- PhD Agriculture Department, Sciences Faculty, Payam-e-Noor University, Tehran, Iran
3- PhD Young Researchers and Elite Club, Mashhad Branch, Islamic Azad University, Mashhad, Iran , Damoon.sadughi@gmail.com
Abstract:   (1549 Views)

Aims: Wound healing delay is a common complication of diabetes mellitus. Considering the antioxidant properties of the Launaea acanthodes, this study aimed to examine the effects of this plant on open skin wound healing in diabetic rats.

Materials & Methods: In this experimental study, 32 male rats were allocated into the equal groups of control, diabetic control and experimental diabetic 1 and 2. The diabetes in diabetic control and experimental diabetic groups was induced using an intraperitoneal injection of alloxan. In all samples, 4 wound with 7mm diameters were created in two lateral posterial parts of body. Then experimental diabetic groups 1 and 2 treated with 10 and 20% of Launaea acanthodes ointment and control and diabetic control groups treated with eucerin for 12 days. Histological studies were performed on each of the days 3, 6, 9 and 12. Data were analyzed by Kruskal-Wallis and post-hoc Tukey tests in SPSS 20 software.

Findings: The highest and lowest density of inflammatory cells in control group are 3th and 12th day, diabetic control 9th and 3th day, experimental diabetic 1 and 2 6th and 12th day. The highest and lowest thickness of the epithelium in control group are 6th and 12th day, diabetic control 12th and 3th day, experimental 1 9th and 12th day and experimental 2 6th and 12th day. The highest and lowest density of blood vessels in control group are 6th and 12th day, diabetic control 12th and 3th day, experimental 1 6th and 3th day and experimental 2 6th and 12th day.

Conclusion: Topical treatment of aqueous extract of Launaea acanthodes accelerates the healing of diabetic ulcers.

Keywords: Wound, Diabetes Mellitus, Skin, Rats
1- Matveyenko A, Vella A. Regenerative medicine in diabetes. Mayo Clin Proc. 2015;90(4):546-54. 2- Adams SB Jr, Sabesan VJ, Easley ME. Wound healing agents. Crit Care Nurs Clin North Am. 2012;24(2):255-60. 3- Bazrafshan A, Owji M, Yazdani M, Varedi M. Activation of mitosis and angiogenesis in diabetes-impaired wound healing by processed human amniotic fluid. J Surg Res. 2014;188(2):545-52. 4- Mott JD, Werb Z. Regulation of matrix biology by matrix metalloproteinases. Curr Opin Cell Biol. 2004;16(5):558-64. 5- Moura LI, Dias AM, Suesca E, Casadiegos S, Leal EC, Fontanilla MR, et al. Neurotensin-loaded collagen dressings reduce inflammation and improve wound healing in diabetic mice. Biochim Biophys Acta. 2014;1842(1):32-43. 6- Oviedo-Socarras T, Vasconcelos AC, Barbosa IX, Pereira NB, Campos PP, Andrade SP. Diabetes alters inflammation, angiogenesis, and fibrogenesis in intraperitoneal implants in rats. Microvasc Res. 2014;93:23-9. 7- Lobmann R, Zemlin C, Motzkau M, Reschke K, Lehnert H. Expression of matrix metalloproteinases and growth factors in diabetic foot wounds treated with a protease absorbent dressing. J Diabetes Complic. 2006;20(5):329-35. 8- Thomas DR. Clinical management of diabetic ulcers. Clin Geriatr Med. 2013;29(2):433-41. 9- Schafer M, Werner S. Oxidative stress in normal and impaired wound repair. Pharmacol Res. 2008;58(2):165-71. 10- Boulton AJ. The pathway to foot ulceration in diabetes. Med Clin North Am. 2013;97(5):775-90. 11- Hajinejad Boshroue R, Behnam Rassouli M, Tehranipour M, Gheybi F, Hajinejad Sh, Elahi Moghaddam Z. The effects of hydro- alcoholic extract of launaea acanthodes on the blood, Urine Albumin and Bilirubin levels in male hyperglycemic wistar rat. Iran J Endocrinol Metab. 2013;15(2):190-233. [Persian] 12- Piazza L, Bertini S, Milany J. Extraction and structural characterization of the polysaccharide fraction of Launaea acanthodes gum. Carbohydr Polym. 2010;79(2):449-54. 13- Karimidokht shahrbabaki A, Oryan Sh, Parivar K. Anticonvulsant activity of ethanolic extract and aqueous fraction of Launaea acanthodes gum in comparison with diazepam in mice. J Qhazvin Univ Med Sci. 2009;13(1):14-20. [Persian] 14- Rohbarian R, Sepehri Moghadam H, Sadoughi SD. Effect of Aqueous Extract of Launaea acanthodes on Testicular Tissue and Sperm Parameters in Alloxan-Induced Diabetic Rats. Q Horiz Med Sci. 2015;21(1):21-9. [Persian] 15- Sepehri-Moghadam H, Rahbarian R, Sadoughi SD. The effect of aqueous extract of Launaea acanthodes (Boiss.) O. Kuntze on the serum level of insulin and blood glucose and histomorphological changes of pancreas in diabetic rats. Feyz. 2015;19(1):30-7. [Persian] 16- Jalali M, Behnam Rassouli M, Tehranipour M, Ghayour N, Khayatzadeh J, Jannati H. Study of the effects of hyperglycemia and Launaea acanthodes extract administration on disorders of liver function in rats. Physiol Pharmacol. 2012;15(4):562-71. [Persian] 17- Behnam-Rassouli M, Ghayour N, Ghayour MM, Ejtehadi MM. Investigating the effects of hydroalcoholic extract of Launaea acanthodes on the serum levels of glucose, insulin, lipids and lipoproteins in stereptozotocin induced type I diabetic rats. Arak Med Univ J. 2012;14(6):48-56. [Persian] 18- Sadoughi SD. Effect of aqueous extract of Ferula assa-foetida’s resin on wound healing of streptozotocin induced diabetic rats. Q Horiz Med Sci. 2013;19(3):129-35. [Persian] 19- Hoseini-Tahmasbi M, Hoseini-Tahmasbi S, Karami-dehkordi A, Delaram M, MalekAnzabi J, Fatahi F. Effect of Arnebia euchroma root extract on burn wound healing in Balb/c mice. J Shahrekord Univ Med Sci. 2013;15(4):54-61. [Persian] 20- Kitano Y, Yoshimura K, Uchida G, Sato K, Harii K. Pretreament of topical all-trans acid is benefical for wound healing in genetically diabetic mice. Arch Dermatol Res. 2001;293(10):515-21. 21- Lateef H, Abatan OI, Nadeem Aslam M, Stevens MJ, Varani J. Topical pretreatment of diabetic rats with all-trans retinoic acid improves healing of subsequently induced abrasion wounds. Diabetes. 2005;54(3):855-61. 22- Ringsdorf WM Jr, Cheraskin E. Vitamin C and human wound healing. Oral Surg Oral Med Oral Pathol. 1982;53(3):231-6. 23- Jagetia GC, Rajanikant GK, Baliga MS, Rao KV, kumar P. Augmentation of wound headling by ascorbic acid treatment in mice exposed to gamma- raditition. Int J Radiat Biol. 2004;80(5):347-54. 24- Pinnel SR, Murad S, Darr D. Induction of Collagen Synthesis by Ascorbic AcidA Possible Mechanism. Arch Dermatol. 1987;123(12):1684-6. 25- Nusgens BV, Humbert Ph, Rougier A, Colige AC, Haftek M, Lambert CA, et al. Topically applied vitamin C enhances the mRNA level of collagens I and III, their processing enzymes and tissue inhibitor of matrix metalloproteinase 1 in the human dermis. J Invest Dermatol. 2001;116(6):853-9. 26- Lukacinova A, Mojzis J, Benacka R, Keller J, Maguth T, Kurila P, et al. Preventive effects of flavonoids on alloxan-induced diabetes mellitus in rats. Acta Vet Brno. 2008;77:175-82. 27- Lodhi S, Singhai AK. Wound healing effect of flavonoid rich fraction and luteolin isolated from Martynia annua Linn on streptozotocin induced diabetic rats. Asian Pac J Trop Med. 2013;6(4):253-9. 28- Zellagui A, Gherraf N, Ladjel S, Hameurlaine S. Chemical composition and antibacterial activity of the essential oils from Launaea resedifolia L. Org Med Chem Lett.. 2012;2(1):2. 29- Tonnesen MG, Feng X, Clark RF. Angiogenesis in wound healing J Investig Dermatol Symp Proc. 2000;5(1):40-6. 30- Wang L, Yang X, Qin P, Shan F, Ren G. Flavonoid composition, antibacterial and antioxidant properties of tartary buckwheat bran extract. Ind Crops Prod. 2013;49:312-7. 31- Feng R, Guo ZG, Yan CM, Li EG, Tan RX, Ge HM. Anti-inflammatory flavonoids from Cryptocarya chingii. Phytochem. 2012;76:98-105. 32- Greaves NS, Ashcroft KJ, Baguneid M, Bayat A. Current understanding of molecular and cellular mechanisms in fibroplasia and angiogenesis during acute wound healing. J Dermatol Sci. 2013;72(3):206-17.
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Type of Study: Original | Subject: Basic Medical Science
Received: 2015/04/27 | Accepted: 2015/11/14 | Published: 2015/11/23
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DOI: 10.18869/acadpub.hms.22.1.1

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Rahbarian R., Sepehri Moghadam H., Sadoughi S.D.. Effect of Aqueous Extract of Launaea acanthodes on Open Skin Wound in Diabetic Rats. Horizon Med Sci . 2016; 22 (1) :1-11
URL: http://hms.gmu.ac.ir/article-1-2196-en.html
Volume 22, Number 1 (Winter 2016) Back to browse issues page
مجله علمی پژوهشی افق دانش The Horizon of Medical Sciences
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