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:: Volume 21, Number 1 (Spring 2015) ::
Horizon Med Sci 2015, 21(1): 13-19 Back to browse issues page
Evaluation of Oxidative Stress Biomarkers in Rat Heart Exposed to Diazinon and Vitamins E and C
Kavous Tahmasebi1, Mahvash Jafari 2, Abbas Ahmadi3
1- MSc Biochemistry Department, Medicine Faculty, Baqiyatallah University of Medical Sciences, Tehran, Iran
2- PhD Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran , Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
3- MSc Students Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Abstract:   (1293 Views)

Aims: Organophosphates such as diazinon (DZN) induce the production of free radicals and oxidative stress. The aim of this study was to evaluate the role of vitamins E and C as antioxidants in reduction of DZN-induced oxidative stress in rat heart. Materials & Methods: In present experimental study, 36 male Wistar rats were randomly divided into six groups control (corn oil as DZN solvent), DZN (100mg/kg), vitamin E (150mg/kg), vitamin C (200mg/kg), vitamin E+DZN and vitamin C+DZN. 24 hours after intraperitoneal injection, animals were anesthetized by ether, and heart tissue was quickly removed. After tissues hemogenation, superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST) and lactate dehydrogenase (LDH) activities, as well as glutathione (GSH) and malondialdehyde (MDA) levels were determined by biochemical methods. The data were statistically analyzed using Instat 3.3 by analysis of variance (ANOVA) followed by post hoc Tukey test. Findings: DZN increased SOD, CAT and GST activities (p<0.01) and MDA level (p<0.05), while decreased LDH activity (p<0.05) and GSH content (p<0.01) in heart compared with the control group. Administration of vitamins E and C inhibited changing of these parameters. Conclusion: Administration of vitamins E and C as antioxidant decreases DZN-induced oxidative stress by scavenging free radicals.

Keywords: Diazinon [http://www.ncbi.nlm.nih.gov/mesh/68003976], Vitamin E [http://www.ncbi.nlm.nih.gov/mesh/68014810], Vitamin C [http://www.ncbi.nlm.nih.gov/mesh/68001205], Oxidative Stress [http://www.ncbi.nlm.nih.gov/mesh/68018384], Heart [http://www.ncbi.nlm.ni
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Type of Study: Original | Subject: Basic Medical Science
Received: 2014/11/12 | Accepted: 2015/01/31 | Published: 2015/03/17
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DOI: 10.18869/acadpub.hms.21.1.13


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Tahmasebi K, Jafari M, Ahmadi A. Evaluation of Oxidative Stress Biomarkers in Rat Heart Exposed to Diazinon and Vitamins E and C. Horizon Med Sci . 2015; 21 (1) :13-19
URL: http://hms.gmu.ac.ir/article-1-2088-en.html
Volume 21, Number 1 (Spring 2015) Back to browse issues page
مجله علمی پژوهشی افق دانش The Horizon of Medical Sciences
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